Design and carry out experiments to explore the neuroprotective ability of soluble FasL in glaucoma and other models of ocular immunity (see PMID: 33565149). We have found that glaucoma results from membrane-bound FasL-induced death of retinal ganglion cells (RGCs), but can be prevented by soluble FasL. We are trying to understand the distinct signaling pathways downstream of Fas following engagement by membrane-bound vs soluble FasL. Studies will involve unique murine models for detection and isolating FasL from experimental and control eyes as well as the capacity of AAV-sFasL injection to prevent and reverse damage to RGCs and other FasL targets. Studies will be a collaboration between the Marshak-Rothstein lab at UMMS and the Gregory-Ksander lab at MEEI.
Expectations also include oral presentations for program/department research in progress meetings, submission of abstracts for poster and oral presentations at national/international meetings, and oversight of technician and/or student working on related projects.
Highly motivated investigator with a PhD in Immunology, Cell Biology, Molecular Biology or related discipline.
Working knowledge of cellular immunology, ocular immunology, cell signaling, and molecular biology.
Hands-on laboratory experience in relevant methodologies, trouble-shooting technical problems, and data analysis skills.
Experience in the following techniques preferred: handling/injecting/genotyping mice, preparation of ocular tissues for histology and confocal microscopy, isolation and cell culture of microglial cells, multicolor flow cytometry, DNA/RNA purification and quantification, qPCR and RNAseq, enzyme-based immunoassays, and database comparisons.
Outstanding organizational and time management skills, as well as the ability to work both independently and as part of a team in a fast-paced environment.
University of Massachusetts Medical Center:
Find out more about the UMMS research community at http://www.umassmed.edu/research-home/
The position includes opportunities for project and career development, interaction with a diverse, talented and collegial immunology community at UMMS.
UMass Medical Center is an equal opportunity employer.
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