University of Massachusetts Medical School

  • Research Lab Tech II

    Minimum Salary US-MA-Worcester
    Job Location 2 weeks ago(11/26/2018 1:09 PM)
    Requisition Number
    2018-31991
    # of Openings
    1
    Posted Date
    Day
    Shift
    Non Exempt
    Exempt/Non-Exempt Status
    Non Union Position-W25-Non Unit Classified
  • Overview

    GENERAL SUMMARY OF POSITION:   

    Under the direct supervision of a Principal Investigator or designee, the Research Lab Tech II performs and interprets routine and highly complex experiments.

     

    Responsibilities

    MAJOR RESPONSIBILITIES: 

    • Performs a variety of laboratory experiments using both routine and special techniques and methods
    • May assist in the training of others
    • Provides input to improve current procedures, protocols, and/or methods
    • Writes standard operating procedures, schedules work, orders supplies, and maintains an adequate inventory of supplies, reagents, etc.
    • Prepares laboratory reagents, buffers, and media
    • Records results, organizes data and performs computations
    • Performs duties independently; requiring action and judgment to solve commonly encountered problems
    • Complies with all safety and infection control standards appropriate to this position
    • Perform other duties as required.

    Qualifications

    REQUIRED QUALIFICATIONS:

    • Bachelor’s degree in a Biological Science or equivalent experience
    • 1 year of related experience
    • Knowledge of Microsoft Office products
    • Ability to perform duties independently
    • Judgment and action skills required to solve commonly encountered problems

    PREFERRED QUALIFICATIONS:

    Experience Required:

    • Experience with zebrafish husbandry.
    • Experience with zebrafish embryo microinjection

    Experience Desired (not required):

    • Basic molecular biology techniques (PCR, Restriction Enzyme mediated cloning, Gel electrophoresis, Qiagen DNA miniprep).
    • Bacteria Strain preparation and handling (competent cell preparation and agar plate preparation).

    SUPERVISION RECEIVED:

    Under the direct supervision of a Principal Investigator or designee

     

    SUPERVISION EXERCISED: 

    None

     

    ENVIRONMENTAL WORKING CONDITIONS:

    Wet chemistry laboratory, and may require appropriate contact with biohazards, radionuclides, toxins, animals, and human specimens. 

    Additional Information

     

    Additional Information:

    Laboratory uses CRISPR/Cas9 tools for the development of gene therapy reagents

     

    Laboratory uses zebrafish as a model organism for two major projects:

    1. developing tools for gene inactivation and modification in vertebrate systems.
    2. creating disease models and studying gene function

    Major Responsibilities (in addition):

    1. Responsible for the maintenance of mutant and transgenic zebrafish lines.
    2. Participate in the construction of Cas9 nuclease variants with increased precision
    3. Isolate and purify DNA from zebrafish embryos and adult finclips.
    4. Maintain the laboratory database and frozen archive of plasmids and bacterial strains.
    5. Prepare competent bacterial cells for transformation and media reagents.
    6. Ordering standard supplies for the laboratory.

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